V5 tagmolecular weight The V5 tag peptide is a crucial tool in molecular biology, widely employed for the detection and purification of recombinant proteins. This short peptide sequence, typically derived from the P and V proteins of Simian Virus 5 (SV5), acts as an epitope that can be genetically fused to a protein of interestV5 Tag Peptide (25mg). Its primary utility lies in enabling the specific binding of antibodies, facilitating downstream applications such as Western blotting, ELISA, and affinity purification. Understanding the properties, applications, and selection criteria for V5 tag peptides is essential for researchers aiming to effectively study protein expression and function.
The V5 tag is a small, highly specific epitope that can be incorporated into recombinant proteins.V5EpitopeTag PeptideTFA is atag peptidederived from a small epitope present on the P and V proteins of the paramyxovirus of simian virus 5. Its origin from Simian Virus 5 (SV5) provides a distinct antigenic site that can be recognized by specific antibodiesV5-tag peptide. This recognition is the cornerstone of its utility. When a protein is expressed with a V5 tag, antibodies raised against this tag can bind to it, allowing for its identification and quantification.
The most common sequence for the V5 tag is a 14-amino acid polypeptide: GKPIPNPLLGLDST. However, shorter versions, such as a 9-amino acid sequence (IPNPLLGLD), have also been utilized.3315-205V5-tag peptide. Code No. 3315-205. V5-tag peptide. Availability (in Japan). 10 or more. （In Japan at 00:05, Feb 21, 2026 in JST）. Size. 2 mg x 5. Data ... The choice of tag length can sometimes influence antibody recognition and overall protein expression, though the 14-amino acid sequence is generally preferred for robust detection.This polyclonal anti-V5-tag antibodydetects over-expressed proteins containing the V5 epitope tag. To date this antibody has reacted with all V5 tagged ...
The V5 tag peptide system offers a versatile solution for various protein research needs:
* Protein Detection: The most straightforward application is detecting the presence of a V5-tagged protein. This is commonly achieved through techniques like Western blotting, where antibodies bind to the V5 tag, and subsequent detection methods (e.V5 Tag Peptide -20575ES - Yeaseng., chemiluminescence) reveal the protein's location and abundance. ELISA assays also leverage V5 tag detection for quantitative analysis of proteins.
* Protein Purification: The V5 tag serves as an excellent handle for affinity purification. Proteins fused to the V5 tag can be selectively captured from complex cellular lysates using immobilized anti-V5 antibodies or affinity resinsOur V5 tag antibodies aredesigned to reliably detect recombinant V5-tagged proteins. Each antibody is validated for use in various applications.. This process allows for the isolation of highly pure V5-tagged proteins, free from other cellular components.
* Competitive Elution: In affinity purification, the V5 tag peptide itself can be used to competitively elute the bound V5-tagged fusion proteins from the antibody-coated beads or resins. By adding a high concentration of free V5 tag peptide, it can displace the tagged protein, allowing for its recovery in a soluble form.
* Nanobody Development: The V5 tag system has also been instrumental in the development of nanobodies specifically designed to target the V5 epitope. These nanobodies offer alternative binding reagents with potentially different properties and applications compared to traditional monoclonal antibodies.
When working with V5 tag peptides and their associated reagents, several factors are important for ensuring successful experiments:
* Purity and Quality: For both V5 tag peptides used for elution and V5 antibodies used for detection, high purity is critical. Impurities can lead to non-specific binding, false positives, or reduced assay sensitivity.The V5-Epitope Tag for Cell Engineering and Its Use in ... - PMC Manufacturers often provide specifications for purity, such as HPLC grade, to indicate quality.
* Antibody Specificity and Reactivity: V5 tag antibodies are designed to recognize the V5 epitope. It's important to select an antibody that has been validated for the specific application (eV5 Epitope Tag Peptideis a peptide tag used in protein researchfor affinity purification and detection of recombinant proteins, with applications in ....g., Western blot, ELISA, immunoprecipitation) and that exhibits reliable reactivity with both N-terminal and C-terminal tagged proteins, if applicable.ELISA(peptide):For ELISA detection of antigenic peptide. Reactivity: All. Clonality: Monoclonal [AFB17768]. Specificity: T0057 detects a small epitope ... Some antibodies may show preferential binding to one orientation.
* Peptide for Elution: When using the V5 tag peptide for competitive elution, its concentration and purity are paramount. The peptide should be of sufficient quality to effectively compete with the tagged protein for antibody binding.V5 tag Antibodies - Sino Biological
* Experimental Context: The choice of V5 tag reagent may also depend on the experimental contextV5 Epitope Tag Antibody (OARA01985). For instance, if working with sensitive downstream assays, using a V5-tag-directed nanobody might offer advantages over a conventional antibody.Bioactivity. Description.V5 Epitope Tag Peptide acetate is a peptide. Chemical Properties. Molecular Weight, 1481.65. Formula, C66H112N16O22.
The V5 tag peptide system remains a cornerstone in protein research, providing a reliable and efficient means to detect, purify, and manipulate recombinant proteinsV5 Epitope Tag Peptide. Its widespread adoption is a testament to its effectiveness and versatility in a variety of molecular biology workflows3315-205V5-tag peptide. Code No. 3315-205. V5-tag peptide. Availability (in Japan). 10 or more. （In Japan at 00:05, Feb 21, 2026 in JST）. Size. 2 mg x 5. Data ....
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