V5-tag nanobody The V5 tag is a widely recognized epitope tag that plays a crucial role in protein research, particularly in molecular biology and biotechnology. At its core, the V5 tag is defined by its specific amino acid sequence, which allows for the detection and purification of tagged proteins. Understanding the V5 tag peptide sequence is essential for researchers utilizing this common tool.V5 Tag Peptide -20575ES - Yeasen
The most commonly cited and utilized sequence for the V5 tag is a 14-amino acid peptide: GKPIPNPLLGLDST. This sequence is derived from an epitope present on the P and V proteins of simian virus 5 (SV5), a paramyxovirusSequence ·Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr; Synonym · V5 Epitope Tag ; Molecular Weight · 1421.67 ; Molecular Formula · C ; H .... Researchers often refer to this sequence in its full length when designing experiments or interpreting resultsThe recognizedV5epitope represents 95GKPIPNPLLGLDST108 of RNA polymerase alpha subunit of simian parainuenza virus type 5. This shortpeptide sequencewas ....
While the 14-amino acid sequence is standard, a shorter version, IPNPLLGLD (9 amino acids), is also sometimes employed, though less frequently. The choice between the full and truncated sequence can depend on specific experimental needs, such as the available antibody binding sites or the desired size of the tag.
The V5 tag's utility stems from several key characteristics:
* Defined Sequence: The specific amino acid sequence (GKPIPNPLLGLDST) is critical for antibody recognitionTheV5 tagis usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9amino acid sequence(IPNPLLGLD).. This precise sequence ensures that antibodies raised against the V5 tag will bind specifically to the tagged protein.
* Epitope Origin: Its origin from simian virus 5 (SV5) provides a biological basis for its selection as a tagIX203: pMVP (L5-L4) V5 epitope tag Sequences (1). The epitope is part of the P and V proteins of this virus.
* Molecular Weight: The 14-amino acid V5 tag has a molecular weight of approximately 1421.Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pcDNA™3.1/V5-His A or pcDNA™3.1/V5-His B.67 Da. This relatively small size means it typically does not significantly interfere with the function or folding of the target protein.
* Versatility: The V5 tag is widely used in various applications, including Western blotting, immunoprecipitation, immunofluorescence, and protein purification. This versatility is supported by the availability of robust V5 tag antibodies.
The primary application of the V5 tag peptide sequence is to enable the specific detection and manipulation of recombinant proteins. Researchers fuse the V5 tag to the N-terminus or C-terminus of a target proteinV5-tag peptide. Subsequently, antibodies specifically designed to recognize the V5 epitope are used.
Common detection methods include:
* Western Blotting: Antibodies against the V5 tag are used to detect the presence and size of the tagged protein after separation by SDS-PAGEV5EpitopeTag Peptideis apeptide tagused in protein research for ...Sequence. GKPIPNPLLGLDST. Purity. ≥95%. Synonyms. H-Gly-Lys-Pro-Ile-Pro-Asn-Pro ....
* Immunoprecipitation (IP): V5-tagged proteins can be isolated from cell lysates using anti-V5 antibodies, facilitating downstream analysis or purification.
* Immunofluorescence/Immunohistochemistry: The V5 tag allows for the visualization of protein localization within cells or tissues.
While the core V5 tag peptide sequence is well-defined, related terms and concepts often appear in discussions:
* V5 Tag Antibodies: A wide range of antibodies are available from various manufacturers, targeting the V5 epitope. These include monoclonal and polyclonal antibodies, often validated for specific applications like ELISA, IP, and Western blotting.
* V5-Tag Peptide: Synthetic V5 tag peptides are commercially available. These peptides often serve as blocking agents in immunoassays to confirm antibody specificity or as standards. Some may be modified, such as with N-terminal biotinylation or cysteine additions (e.g.8SKJ: Crystal structure of a Nanobody bound to the V5 ..., N-Cys-GKPIPNPLLGLDST).
* His-Tag: Another common epitope tag, the His-tag (often a polyhistidine sequence), is frequently used in conjunction with the V5 tag, creating a V5-His tag. This dual-tagging strategy allows for orthogonal purification and detection methods. Mammalian vectors are often designed to express proteins with both tags.V5-Trap for immunoprecipitation of V5-tagged proteins - LubioScience
* V5-Tag Purification: Techniques like affinity chromatography using anti-V5 antibodies or resins are employed for purifying V5-tagged proteins.
* V5-Tag Size: While the 14-amino acid sequence is standard, the concept of tag size is relevant, with the shorter 9-amino acid variant being a notable alternative.This peptide is biotinylated at the N-terminus. V5 Tag - GKPIPNPLLGLDST - Specifications.Peptide sequence: H-GKPIPNPLLGLDST-OH; Amount: 5 mg; Purity: Trial ...
The V5 tag peptide sequence, primarily GKPIPNPLLGLDST, is a fundamental element in modern molecular biology research.V5 Epitope Tag Peptide TFAis a tag peptide derived from a small epitope present on the P and V proteins of the paramyxovirus of simian virus 5. Its well-defined nature, coupled with the availability of specific antibodies and its compatibility with various experimental techniques, makes it an indispensable tool for protein expression, detection, and purification. Understanding the sequence, its origin, and related applications ensures effective utilization of this powerful epitope tag.
Join the newsletter to receive news, updates, new products and freebies in your inbox.